FIG 5.

Multiplex lateral flow immunoassay. Bacteria were grown at 28°C and used at 10 times the limit of detection. The bacterial suspensions were incubated for 10 min with a mix of colloidal gold-labeled Ye18*, Ye300*, Yps 104*, and Yp7* MAbs, and the LFI dipsticks were then dipped for 30 min into 100 μl of the bacterial suspensions for upward migration of the liquid. The Ye4 and Ye18 MAbs were used for the test line of Y. enterocolitica 2/O:9 and Y. enterocolitica 4/O:3, respectively. A mix of Yps103 and Yp7 was used for the test line of Y. pseudotuberculosis.