TABLE 2.
Primer sequences used in the PCR assay for the detection of major E. coli STs
| Primer | Sequence | Size (bp) | Positions | Region, ST, E. coli strain (GenBank accession no.) |
|---|---|---|---|---|
| ST73_for | TGGTTTTACCATTTTGTCGGA | 490 | 2001936–2001916 | 1, ST73, CFT073 (AE014075) |
| ST73_rev | GGAAATCGTTGATGTTGGCT | 2001447–2001466 | ||
| ST131_for | GACTGCATTTCGTCGCCATA | 310 | 4344866–4344847 | 19, ST131, NA114 (CP002797) |
| ST131_rev | CCGGCGGCATCATAATGAAA | 4344565–4344584 | ||
| ST95_for | ACTAATCAGGATGGCGAGAC | 200 | 3124468–3124487 | 9, ST95, UTI89 (CP000243) |
| ST95_rev | ATCACGCCCATTAATCCAGT | 3124668–3124649 | ||
| ST69_for | ATCTGGAGGCAACAAGCATA | 104 | 3053203–3053222 | 21, ST69, UMN026 (CU928163) |
| ST69_rev | AGAGAAAGGGCGTTCAGAAT | 3053306–3053287 |