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hla gene prevalence, hemolytic activity, and alpha-toxin expression in S. aureus isolates. Assessment of hla gene prevalence by PCR (A), in vitro hemolytic activity by quantitative analysis of rabbit RBC lysis (B), and alpha-toxin expression measurement via a capture ELISA and Western blotting (C) was performed for S. aureus isolates obtained from SAB subjects with either end-stage renal disease requiring chronic hemodialysis or who had had a surgical procedure in the preceding 30 days.
