TABLE 1.

In vitro susceptibilities of 187 isolates of Candida spp. to amphotericin B, fluconazole, voriconazole, and posaconazole^a

Species (no. of isolates tested)	Antifungal agent	Test method	MIC (μg/ml)		EA (%)	CA (%)	% errors
			Range	Mode			VME	ME	Minor errors
C. albicans (120)	Amphotericin B	BMD	0.25–2	1
	Amphotericin B	MIC test strip	0.125–0.75	0.38	81.67	98.3	1.7	0	0
	Amphotericin B	SensiQuattro	<0.5–>2	0.5	100	97.4	1.7	0.9	0
	Fluconazole	BMD	0.12–>64	0.12
	Fluconazole	MIC test strip	0.064–>256	0.38	86.67	91.7	3.3	2.5	2.5
	Fluconazole	SensiQuattro	<1–>8	1	45.7	94	3.4	2.6	0
	Voriconazole	BMD	0.016–>8	0.016
	Voriconazole	MIC test strip	0.004–0.125	0.016	96.67	97.5	2.5	0	0
	Voriconazole	SensiQuattro	<0.06–>0.5	0.06	93.67	94.8	2.6	2.6	0
	Posaconazole	BMD	0.016–>8	0.06
	Posaconazole	MIC test strip	0.016–3	0.064	95.8	91.7	3.3	5	0
	Posaconazole	SensiQuattro	<0.03–>0.25	0.03	94.83	93.2	3.4	3.4	0
C. glabrata (38)	Amphotericin B	BMD	0.25–2	1
	Amphotericin B	MIC test strip	0.19–1	0.75	94.5	86.80	13.2	0	0
	Amphotericin B	SensiQuattro	<0.5–2	0.5	100	84.2	7.9	7.9	0
	Fluconazole	BMD	0.5–>64	4
	Fluconazole	MIC test strip	2–256	8	97.4	97.40	0	0	2.6
	Fluconazole	SensiQuattro	<1–>8	8	65.8	ND	ND	ND	ND
	Voriconazole	BMD	0.016–4	0.25
	Voriconazole	MIC test strip	0.032–32	0.094	89.5	94.70	0	5.3	0
	Voriconazole	SensiQuattro	0.06–>0.5	0.5	60.5	28.4	0	81.6	0
	Posaconazole	BMD	0.12–>8	0.5
	Posaconazole	MIC test strip	0.125–12	0.5	92	92.10	0	7.9	0
	Posaconazole	SensiQuattro	>0.25	0.25	90	ND	ND	ND	ND
C. parapsilosis (10)	Amphotericin B	BMD	0.5–2	1
	Amphotericin B	MIC test strip	0.094–0.5	0.25	60	90	10	0	0
	Amphotericin B	SensiQuattro	0.5	0.5	100	90	10	0	0
	Fluconazole	BMD	0.25–4	0.5
	Fluconazole	MIC test strip	0.5–4	0.5	90	100	0	0	0
	Fluconazole	SensiQuattro	1	1	100	100	0	0	0
	Voriconazole	BMD	0.016–0.25	0.016
	Voriconazole	MIC test strip	0.004–0.19	0.19	80	70	10	20	0
	Voriconazole	SensiQuattro	<0.06	0.06	100	90	10	0	0
	Posaconazole	BMD	0.03–0.12	0.06
	Posaconazole	MIC test strip	0.023–0.25	0.094	100	80	0	20	0
	Posaconazole	SensiQuattro	<0.03	0.03	90	90	10	0	0
C. tropicalis (12)	Amphotericin B	BMD	0.5–1	1
	Amphotericin B	MIC test strip	0.19–0.75	0.25	83.3	100	0	0	0
	Amphotericin B	SensiQuattro	0.5	0.5	100	100	0	0	0
	Fluconazole	BMD	0.12–4	0.5
	Fluconazole	MIC test strip	0.25–4	0.38	100	100	0	0	0
	Fluconazole	SensiQuattro	<1–>8	1	50	58.3	0	41.7	0
	Voriconazole	BMD	0.016–0.03	0.016
	Voriconazole	MIC test strip	0.012–0.047	0.016	100	66.7	0	33.3	0
	Voriconazole	SensiQuattro	<0.06–>0.5	0.06	66.7	66.7	0	33.3	0
	Posaconazole	BMD	0.016–0.25	0.06
	Posaconazole	MIC test strip	0.008–0.125	0.064	100	66.7	0	33.3	0
	Posaconazole	SensiQuattro	<0.03–0.25	0.03	66.7	66.7	0	33.3	0
C. krusei (7)	Amphotericin B	BMD	1.0–2	2
	Amphotericin B	MIC test strip	0.125–2	1	100	42.9	57.1	0	0
	Amphotericin B	SensiQuattro	0.5	0.5	100	28.6	71.4	0	0
	Fluconazole	BMD	16–>64	16
	Fluconazole	MIC test strip	16–>256	32	100	100	0	0	0
	Fluconazole	SensiQuattro	<1–>8	8	85.7	ND	ND	ND	ND
	Voriconazole	BMD	0.25–2	0.25
	Voriconazole	MIC test strip	0.038–3	0.19	85.7	85.7	14.3	0	0
	Voriconazole	SensiQuattro	<0.06–0.25	0.06	57.1	71.4	28.6	0	0
	Posaconazole	BMD	0.03–1	0.25
	Posaconazole	MIC test strip	0.19–0.75	0.25	100	100	0	0	0
	Posaconazole	SensiQuattro	0.03	0.03	28.6	100	0	0	0

^aAs determined with the SensiQuattro Candida EU method (SQ), the EUCAST broth microdilution (BMD) protocol, and the MIC test strip. EA, essential agreement; CA, categorical agreement; VME, very major error; ME, major error. ND, nondeterminable: measurements of MIC cannot differentiate between susceptible, intermediate, or resistant.