Fig 2.
(A) A Western blot is shown using BMP-2 antibody-assessed protein production of rBMCs transduced with AdBMP-2Myc. Lane A represents recombinant human BMP-2 (60 ng). Lanes B and C contained the conditioned medium from rBMCs infected with Adβ-gal and AdBMP-2Myc, respectively. The 30-kDa band was detected on Lanes A and C by the BMP-2 antibody under nonreducing conditions (black arrow). No signal was detected from Lane B. (B) The same membrane also was analyzed with the Myc antibody after pretreated BMP-2 antibodies were completely stripped. In Lane C the same band, which was detected by BMP-2 antibodies, also was detected by the Myc antibody confirming production of virally mediated, epitope-tagged BMP-2.