Skip to main content
. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Clin Immunol. 2014 Nov 11;156(1):65–73. doi: 10.1016/j.clim.2014.11.003

Figure 3.

Figure 3

PTPN22 is necessary for efficient IFNα production by pDCs. Splenocytes were stimulated with either Imiquimod (IMQ) or R848 for 4 hours in vitro. The cells were then fixed and permeabilized and stained for IFNα. A) Representative flow cytometry plots of IFNα expression on B6 pDCs (gated on CD19- B220+ PDCA-1+). B) Shows combined data of B6 PTPN22 WT and KO IFNα+ pDCs. C) PTPN22 KO or WT BXSB-Yaa (younger than 2 months) splenocytes were stimulated for 4 hours in vitro and IFNα was stained and analyzed by flow cytometry. D) Shows combined data from PTPN22 WT and KO BXSB-Yaa mice. Each data point represents 1 mouse. Figure shows results of 2 independent experiments. * p<0.05.