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. 2014 Sep 5;24(2):182–189. doi: 10.1089/scd.2014.0270

FIG. 2.

FIG. 2.

Forced expression of Mixl1 until the epiblast stage is necessary for endodermal induction. (A) Degrees of chimerism in embryonic endodermal tissues with varying periods of Dox administration. Chimerisms were analyzed at E9.5. After anti-EpCAM antibody staining, DsRed-expressing cells were sorted into EpCAM-expressing (EpCAM+) and EpCAM-lacking (EpCAM−) fractions by flow cytometry. Diamonds indicate values calculated by dividing percentage of chimerism in the EpCAM+ fraction by percentage of chimerism in the EpCAM− fraction for individual chimeric embryos. (B) Chimeric fetuses at 1 week after embryo transfer of CHT5- or OTiV1-ESC–injected wild-type blastocysts. In each image, the fetus at the viewer's left grew in the presence (+) of Dox and the fetus at the viewer's right grew in the absence (−) of Dox. (C) Degrees of chimerism in embryonic endodermal tissues in Dox(+) and Dox(−) settings. Triangles indicate values for individual chimeric embryos.