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. Author manuscript; available in PMC: 2015 Jan 12.
Published in final edited form as: Mucosal Immunol. 2013 May 8;7(1):101–113. doi: 10.1038/mi.2013.28

Figure 3. IL-22BP staining on sorted subsets of rat splenic conventional DC.

Figure 3

The two subsets of rat conventional spleen DCs were isolated by cell sorting and let to adhere on poly-L-Lysine pre-coated slides for 30 min in the presence of Brefeldin A and monensin. Slides were then stained with a goat anti-rat IL-22BP pAb followed by a donkey anti-goat IgG-Alexa fluor 568. Controls were performed using the secondary antibody alone. Data are representative of two independent experiments.