Figure 7. Retinoic acid is a potent inducer of IL-22BP expression by DCs.
Human monocytes from peripheral blood of healthy donors were differentiated into DCs in complete medium with GM-CSF and IL-4 for 6 days. When indicated, ligands were added at day 4 of culture. On day 6, IL-22BP gene expression was analyzed by RT-qPCR. Bars represent mean ± SEM ratio of IL-22BP gene to HPRT expression as determined by the 2−ΔΔCt method of relative quantification from 7 independent experiments ** p<0.01; * p<0.05.