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. Author manuscript; available in PMC: 2015 Jan 12.
Published in final edited form as: Immunity. 2012 Nov 21;37(6):1050–1060. doi: 10.1016/j.immuni.2012.11.001

Figure 2. IL-34 Is Expressed by Keratinocytes in the Epidermis and Controls the Development of LCs.

Figure 2

(A) Quantitative RT-PCR analysis of Il34 mRNA expression of liver, brain, lung, spleen, and skin in WT mice. Data were normalized to the expression of RNA polymerase 2 (n = 2). Shown is one representative of three individual experiments.

(B) Quantitative RT-PCR analysis of Il34 and Csf1 mRNA expression of WT epidermis and dermis. Data were normalized to the expression of RNA polymerase 2 (n = 2). One representative of two individual experiments is shown.

(C) X-gal staining (blue) of skin sections from Il34LacZ/+ and Il34+/+ mice (scale bar represents 40 mm). HF, Hair follicle.

(D) Skin sections of Il34+/+ mice and Il34LacZ/+ mice were stained with X-gal (blue) and mAb against K14 (red) (scale bar represents 20 μm).

(E) Plots show the percentage and total cell number (±SEM) of LCs (CD11b+MHCII+) among CD45+ epidermal cells in adult Il34LacZ/LacZ and Il34LacZ/+ mice (n = 5). Pooled data of several individual experiments. *p < 0.05, **p < 0.01, ***p < 0.001 (Student's t test, unpaired).

(F) Images display anti-MHCII (LCs) and anti-CD3 (DETCs) staining on ear-derived epidermal sheets from adult Il34LacZ/LacZ and Il34LacZ/+ mice (scale bar represents 50 μm).