(A) Quantitative RT-PCR analysis of Il34 and Csf1 mRNA expression of human epidermis and dermis. Data were normalized to the expression of GAPDH. One of two independent experiments is shown.
(B) Human skin sections were stained with mAbs against K14 and IL-34 (or isotype, mouse IgG1) and counterstained with DAPI (scale bar represents 50 μm).
(C) Human brain sections of the temporal lobe were stained with mAbs against IL-34 (or isotype, mouse IgG1) and NeuN and counterstained with Dapi (scale bar left represents 20 μm, scale bar right represents 5 μm).