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. Author manuscript; available in PMC: 2015 Jan 12.
Published in final edited form as: J Immunol. 2009 Mar 1;182(5):2628–2640. doi: 10.4049/jimmunol.0802954

FIGURE 2.

FIGURE 2

Leukocyte infiltration in WT and GFAP-IκBα-dn spinal cords following EAE. A, Immunofluorescent labeling of WT and GFAP-IκBα-dn tissue sections at 20 dpi (acute disease). B, Immunofluorescent labeling of WT and GFAP-IκBα-dn tissue sections at 40 dpi (chronic disease). Red: Aquaporin-4 (localized to astrocyte end-feet in contact with the vascular endothelium). Green: pan-leukocyte marker CD45. Blue: astrocyte-specific marker GFAP. Scale bar: 100 μm. C, Quantification of CD45 protein expression in spinal cord tissue of WT and GFAP-IκBα-dn mice at 40 dpi. Data are normalized to β-actin. Results represent the mean ± SEM of six animals/group and are expressed as percentage of WT. *, p < 0.05; Student's t test. D, Representative Western blot probed for CD45 and β-actin as loading control.