FIG. 3.

(A) Histology of a cultured ID8 CS. Hematoxylin–eosin (H&E) stain for a representative ID8 monolayer CS. After 24 h of culturing, tight junctions were noted between cells. Cell morphology revealed an irregular cellular morphology with nuclear atypia. Scale bar is 25 μm (×400). (B) Murine CS-derived epithelial ovarian cancer (EOC) tumor versus human stage III EOC tumor. (a) H&E stain of a resected mouse ovary at 1 week after ID8 CS transplantation. Scale bar is 50 μm (×200). Cancerous infiltration of the CS into ovarian parenchyma could be identified (area inscribed with block line). Cell morphology resembled (b) human EOC, stage III (H&E). Scale bar is 50 μm (×200). (c) At 8 weeks post-transplantation, ID8 CS tumors had partially invaded the ovary and replaced original ovarian structure, whereas (d) ICS tumors were still confined to the ovarian bursa. Scale bar is 200 μm (×40), small window; scale bar is 20 μm (×400). Tumor volumes at 8 weeks were 30.712±18.800 mm³ versus 55.837±10.711 mm³ for ICS and CS, respectively, p=0.0990 (n=5). (e) Twelve weeks post-transplantation, the ID8 CS tumors occupied almost the entire ovary. Scale bar is 500 μm (×20), small window; scale bar is 20 μm (×400). (f ) After 12 weeks of ICS tumor growth, the entire ovary showed malignant histology and tumor volumes were significantly larger (283.953±71.676 mm³) than ICS tumors (128.129±44.018 mm³), p=0.0112 (n=5). Scale bar is 500 μm (×20), small window; scale bar is 20 μm (×400). Color images available online at www.liebertpub.com/tec