FIG. 1.

Injury severity and righting reflex prior to control or methylene blue (MB) intervention. (A) Diagram of the experimental study design showing that mice were subjected to a sham injury (Sham) or traumatic brain injury (TBI) at day 0 and immediately (15–30 min later) provided with control of MB interventions. Rotarod acclimation and training were performed prior to injury. Several biochemical and behavioral analyses were performed 1 h – 7 d post-injury. (B) Injury severity was determined by the electromotive force induced by the fluid pulse onto the brain (mV; n=28–29). (C) Time for mice to self-right after sham injury or TBI. Bars represent the mean±standard error of the mean. Means with (*) are significantly different (p<0.05) from Sham-vehicle control (Con).