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. 2015 Jan 15;32(2):127–138. doi: 10.1089/neu.2014.3514

FIG. 3.

FIG. 3.

Methylene blue (MB) intervention reduced the percentage of myeloid (CD11b+/GR1+) cells in circulation after traumatic brain injury (TBI) but had no effect on the percent of brain-associated macrophages. Adult male (3 months old) BALB/c mice were subjected to sham injury (Sham) or TBI and within 15–30 min received an intravenous injection of vehicle control (Con) or MB (2mg/kg). At 1 d post-injury, brain and blood were collected for flow cytometry analysis. (A) Representative bivariate dot plots of CD11b/GR1 labeling of blood leukocytes. (B) Percent CD11b+/GR1+ myeloid cells in circulation (n=8–12). (C) Representative bivariate dot plots of CD11b/CD45 labeling of brain-associated macrophages. (D) Percent CD11b+/CD45hi macrophages associated with the brain (n=8–12). (E) Representative bivariate dot plots from Sham and TBI of CD11b/Ly6C labeling of brain-associated macrophages. (F) Total relative number of Ly6Chi macrophages associated with the brain (out of 10,000 live cells; n=8–12). Bars represent the mean±standard error of the mean. Means with (*) are significantly different (p<0.05) than Sham-Con.