. 2015 Jan 15;32(2):127–138. doi: 10.1089/neu.2014.3514

Table 2.

MB Altered the Immune Profile of LPS-Activated BV2 Microglia

	Saline-Con	Saline-MB	LPS-Con	LPS-MB
CD14	1.07±0.16^a	0.97±0.06^a	1.69±0.12^b	2.97±0.35^c
IL-1β	1.02±0.10^a	0.52±0.16^b	113.7±35.3^c	74.8±6.5^d
TNF-α	1.00±0.04^a	0.54±0.20^b	6.10±1.17^c	4.84±0.59^c
iNOS	1.02±0.10^a	1.03±0.20^a	518.4±139.1^b	551.7±91.6^b
Arg1	1.01±0.10^a	0.91±0.04^a	0.32±0.01^b	0.54±0.08^c
IL-10	1.03±0.11^a	2.14±0.65^a	7.28±0.46^b	17.85±4.05^c

BV-2 microglia were cultured in vitro and treated with saline or LPS (100 ng/mL) for 1 h. After 1 h, control (double deionized water) or methylene blue (MB; 4.5 μM) was added to the culture media. After 24 h, messenger ribonucleic acid (mRNA) was isolated and gene expression of CD14, interleukin (IL)-1β, tumor necrosis factor (TNF)-α, inducible nitric oxide synthase (iNOS), arginase (Arg1), and IL-10 was determined (two independent experiments; n=3 per experiment). Values represent the mean±standard error of the mean. Means with different letters (a,b,c) are significantly different (p<0.05) from each other. Means with underlined letters (d) tend to be different (p=0.1) from lipopolysaccharide (LPS)-vehicle control (Con).