Figure 2. Oxidoreductase activity of the CLIC proteins.

Oxidoreductase enzymatic activity was measured using 5 uM of CLIC proteins or HcTrx-5 or Grx-1, 250 uM NADPH, 1 mM HEDS and 50 nM GR. The reaction was initiated by the addition of 1 mM GSH and the absorbance of NADPH was monitored at A_{340 nm}. Reaction conditions: 5 mM potassium phosphate with 1 mM EDTA, pH 7, at 37°C. (A) Activity of CLIC1, CLIC2 and CLIC4 compared to HcTrx-5 and Grx-1 (positive controls). (B) Activity of 5 uM CLIC1 dimer compared to 5 uM CLIC1 monomer. Error bars represent the S.E. of at least three independent measurements.