Figure 6. Effect of chloride ion channel inhibitor drugs on the oxidoreductase enzymatic activity of CLIC1.

5 uM of CLIC1 reduced (WT) or HcTrx-5 protein was incubated with 10 uM IAA-94, A9C, DIDS or Saxitoxin for ∼1 hour prior use of the protein in the assay. The enzyme assay mixture contained 250 uM NADPH, 1 mM HEDS, 50 nM GR in 5 mM potassium phosphate buffer with 1 mM EDTA, pH 7, at 37°C. The consumption of NADPH was monitored at A_{340 nm} post addition of 1 mM GSH. Error bars shown represent the S.E. of at least three experimental measurements.