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. 2015 Jan 12;10(1):e115699. doi: 10.1371/journal.pone.0115699

Figure 7. Effect of cholesterol on the enzymatic activity of CLIC1.

Figure 7

5 uM of CLIC1 monomer (WT) protein was incubated with 0.4, 0.8 and 1.6 mM cholesterol for ∼1 hour prior to its addition to a reaction mixture of 250 uM NADPH, 1 mM HEDS, 50 nM GR in 5 mM potassium phosphate buffer with 1 mM EDTA, pH 7, at 37°C. The consumption of NADPH was monitored at A340 nm post addition of 1 mM GSH. Control included all the reaction components including 1.6 mM cholesterol, except with no CLIC1 protein. The error bars shown represent the S.E. of at least three experimental measurements.