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. 2015 Jan 12;10(1):e0115111. doi: 10.1371/journal.pone.0115111

Figure 6. β-TrCP interacts with and ubiquitinates PAPC and is required for normal Xenopus embryogenesis.

Figure 6

(A) β-TrCP tagged with RFP co-localized with PAPC-GFP in animal cap cells, both on the plasma membrane and cytoplasmic punta (arrowheads). (B) Expression of TrCPΔF, a dominant-negative form of TrCP that lacks the F-box essential for E3 ligase complex formation, induced PAPC cytoplasmic aggregates in animal cap cells. Note the cells that appear rounder than those in (A). (C) GFP-tagged PAPCΔC was co-expressed with TrCPΔF in Xenopus DMZ cells. (D) TrCPΔF expression inhibited animal cap elongation when treated with activin. TrCPΔF also gave rise to gastrulation phenotypes such as defective blastopore closure and a shortened body axis.