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. 2014 Oct 16;70(2):463–469. doi: 10.1093/jac/dku403

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© The Author 2014. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Clinically observed substitutions alter the stability of NDM variants. (a) Normalized CD spectra of NDM variants. (b) Thermal stability melting curves. (c) Melting temperatures of the NDM variants as determined by CD: NDM-1, 59.5 ± 0.1; NDM-3, 63.4 ± 0.1; NDM-4, 61.4 ± 0.2; NDM-5, 64.6 ± 0.3; NDM-6, 55.1 ± 0.3; NDM-7, 64.6 ± 0.2; and NDM-8, 72.1 ± 0.4. Data are the means of triplicate experiments, with error bars showing the standard deviation (±SD).