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. 2014 Sep 26;24(3):727–739. doi: 10.1093/hmg/ddu491

Figure 8.

Figure 8.

(A–F) Delayed maturation of cultured Sacs−/− spinal motor neurons. (A) Delay in the percentage of motor neurons having reached diameter ≥20 µm and (B) in loss of the developmentally regulated NF protein, peripherin, in dissociated spinal cord cultures from Sacs−/− embryos. (C–F) Anti-NFH immunolabeling of DIV21 (C and D) and DIV42 (E and F) cultures showing accumulation of NF bundles (arrows) in Sacs−/− motor neurons. (G–M) Defects in mitochondrial transport and morphology in cultured Sacs−/− motor neurons. The percentage of motile mitochondria (versus static) is decreased in motor neuron axons in Sacs−/− compared with Sacs+/+ spinal cord cultures at DIV21 (G) and DIV35 (I). Average speed of mitochondrial movement (µm/s) was comparable in Sacs−/− and Sacs+/+ motor neuron axons in DIV21 (H) and DIV35 (J) cultures (5–10 mitochondria per condition). (K–M) Increased mitochondrial length in DIV35 Sacs−/− cultures. Representative images of mitochondrially targeted dsRed in motor neuron axons in DIV21 (K) and DIV35 (L) Sacs+/+ or Sacs−/− cultures. (M) Mean mitochondrial length ± SEM in motor neuron axons in 21 or 35 DIV Sacs+/+ or Sacs−/−cultures. n = 38–49 mitochondria per condition; *Significantly different from Sacs+/+, P < 0.05 (one-way ANOVA with Tukey HSD post hoc analysis).