Figure 4.
Reduced motor neuron survival in Aptx−/− SOD1G93A mice. Spinal cords were harvested from ∼3-month-old wild-type (WT) Aptx−/−, SOD1G93A Aptx−/− SOD1G93A mice and lumbar regions were preserved in 3% neutral buffered formalin, embedded in paraffin blocks, and 10 μm sections were subjected to Nissl staining to quantify the number of motor neurons. A representative image is shown in (A). (B) The number of motor neurons from 40 sections was quantified from three independent littermates and data represent the average ± SEM. Statistical significance between SOD1G93A and Aptx−/− SOD1G93A motor neuron counts (P = 0.026) was calculated using student t-test.