FIG 7.

Carboxymefloquine induces the protein expression of CYP2B6. Primary human hepatocyte cultures from 3 donors were each treated with 30 μM rifampin (RIF) or the indicated doses of carboxymefloquine (CMQ) for 48 h. Cytochrome P450 and β-actin protein expression were analyzed in total protein homogenates by immunoblotting using specific antibodies. (A) Representative Western blot analysis of a single culture. (B) Fold induction of protein expression by chemical treatment was calculated compared to the expression levels in the respective cells treated with DMSO only, which was designated 1 and is indicated by the broken line. The symbols denote cultures from individual donors.