TABLE 1.
Intracellular trypanothione, glutathione, and spermidine levels in Leishmania promastigotes
| Metabolite | Metabolite concentration (μM)a |
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|---|---|---|---|---|---|
| Control | With hypericin (18 μM) treatment |
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| Without supplementation |
With trypanothione (0.5 μM) supplementation |
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| 12h | 24 h | 12 h | 24 h | ||
| Thiols | |||||
| Trypanothione | 317 ± 54 | 43 ± 2 | 39 ± 9 | 235 ± 88 | 478 ± 212 |
| Glutathione | 206 ± 17 | 739 ± 149 | 549 ± 21 | 208 ± 114 | 395 ± 123 |
| Spermidine | |||||
| Without spermidine supplementation | 34 ± 1 | 3 ± 1 | 1 ± 0.1 | 3 ± 0.5 | 2 ± 0.2 |
| With spermidine (100 μM) supplementation | 7 ± 1 | 38 ± 8 | |||
a
The intracellular thiol levels represent concentrations in a final volume of 350 μl containing cell lysates of 1 × 107 Leishmania promastigotes. The intracellular spermidine levels indicate concentrations of spermidine in a final volume of 200 μl containing cell lysates of 1 × 106 Leishmania promastigotes. The control shows concentrations without any treatment. Other data show concentrations with or without supplementation with treatment with hypericin (18 μM).