TABLE 1.
Strain or plasmid | Relevant properties | Reference or source |
---|---|---|
Strains | ||
E. faecium | ||
Aus0004 | Reference strain | 14 |
HM1070 | Reference strain | 15 |
AusTig | In vitro mutant from Aus0004 with reduced susceptibility to TIG | This study |
HMtig1 | In vitro mutant from HM1070 with reduced susceptibility to TIG | This study |
HMtig2 | In vitro mutant from HM1070 with reduced susceptibility to TIG | This study |
EF16 | Clinical isolate with reduced susceptibility to TIG | This study |
EF22 | Clinical isolate with reduced susceptibility to TIG | This study |
AusΔ1228 | Knockout mutant for efau004_1228 from Aus0004 | This study |
E. coli EC1000 | E. coli cloning host | 16 |
Plasmids | ||
pWS3 | 20 | |
pWS3-rpsJ | ΔrpsJ allele cloned into pWS3 | This study |
pWS3-1228 | Δefau004_1228 allele cloned into pWS3 | This study |