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. 2014 Dec 23;59(1):239–244. doi: 10.1128/AAC.04174-14

TABLE 1.

Bacterial strains and plasmids used in the study

Strain or plasmid Relevant properties Reference or source
Strains
    E. faecium
        Aus0004 Reference strain 14
        HM1070 Reference strain 15
        AusTig In vitro mutant from Aus0004 with reduced susceptibility to TIG This study
        HMtig1 In vitro mutant from HM1070 with reduced susceptibility to TIG This study
        HMtig2 In vitro mutant from HM1070 with reduced susceptibility to TIG This study
        EF16 Clinical isolate with reduced susceptibility to TIG This study
        EF22 Clinical isolate with reduced susceptibility to TIG This study
        AusΔ1228 Knockout mutant for efau004_1228 from Aus0004 This study
    E. coli EC1000 E. coli cloning host 16
Plasmids
    pWS3 20
    pWS3-rpsJ ΔrpsJ allele cloned into pWS3 This study
    pWS3-1228 Δefau004_1228 allele cloned into pWS3 This study