TABLE 2.
Primer name | Sequence (5′–3′)a |
Function | |
---|---|---|---|
Forward | Reverse | ||
1228_Seq | TGAGGAAGGTGTGGATCTGA | GCAATGGGTTGAAGGGATAA | Sequencing |
RpsJ_Seq | AGAGGTTGCGACACGCCCGG | TCTACAACAGTTACTGGAAT | Sequencing |
1636_Seq | GTGGAACATAGCGAAAGTATT | TCATGCGGTGGCGCACCTCC | Sequencing |
2455_Seq | GATGAAAGAAGGATCTACTATG | ACTGACAGCTTCCAAGGAGC | Sequencing |
RpsJ_U | AAAGGATCCTACGCTGAAGCTGGAGACAACATTGG | AATGGTACCGTTTTGCCATCTTGTTCCCTCCTTC | Cloning in pWS3 |
RpsJ_D | TAAGGTACCAACTAAAACAAAAATAATGGAGGTG | AAAACTAGTACGATTGCATCGTAAACAACACTTTC | Cloning in pWS3 |
1228_U | CCGGGGATCCGAATTCAAAATCATGGATTACATTTTCTC | TCGTCGTTACTGCTACAGACAAATCCCAATACCAA | Cloning in pWS3 |
1228_D | TAGCAGTAACGACGATCGT | GCAGGTCGACGAATTCAAATCTGCTTGTCTTCTGCA | Cloning in pWS3 |
pWS3 | TTTCCCAGTCACGACGTTGT | ACTGACAGCTTCCAAGGAGC | Cloning verification |
Tet(S) | GACTGTGAATCTAAATTTGAAACC | GCACAATTTCGTGAGTTACTGT | PCR detection |
Tet(T) | TAGCACATGTTGATGCAGGT | TATCATCCCTTACATTTGTC | PCR detection |
Tet(U) | CAAAAGAAATCGATACGTGG | CGTCTGCAGATTCCTTAAAAGTC | PCR detection |
Tet(L) | CATTTGGTCTTATTGGATCG | ATTACACTTCCGATTTCGG | PCR detection |
Tet(M) | ACAGAAAGCTTATTATATAAC | TGGCGTGTCTATGATGTTCAC | PCR detection |
Tet(K) | TATTTTGGCTTTGTATTCTTTCAT | GCTATACCTGTTCCCTCTGATAA | PCR detection |
Tet(O) | GATGGCATACAGGCACAGAC | CAATATCACCAGAGCAGGCT | PCR detection |
Tet(X) | CAATAATTGGTGGTGGACCC | TTCTTACCTTGGACATCCCG | PCR detection |
The underlined sequences correspond to restriction enzyme recognition sites (BamHI, GGATCC; KpnI, GGTACC; EcoRI, GAATTC).