TABLE 2.
Primers used in this study
| Primer name | Sequence (5′–3′)a |
Function | |
|---|---|---|---|
| Forward | Reverse | ||
| 1228_Seq | TGAGGAAGGTGTGGATCTGA | GCAATGGGTTGAAGGGATAA | Sequencing |
| RpsJ_Seq | AGAGGTTGCGACACGCCCGG | TCTACAACAGTTACTGGAAT | Sequencing |
| 1636_Seq | GTGGAACATAGCGAAAGTATT | TCATGCGGTGGCGCACCTCC | Sequencing |
| 2455_Seq | GATGAAAGAAGGATCTACTATG | ACTGACAGCTTCCAAGGAGC | Sequencing |
| RpsJ_U | AAAGGATCCTACGCTGAAGCTGGAGACAACATTGG | AATGGTACCGTTTTGCCATCTTGTTCCCTCCTTC | Cloning in pWS3 |
| RpsJ_D | TAAGGTACCAACTAAAACAAAAATAATGGAGGTG | AAAACTAGTACGATTGCATCGTAAACAACACTTTC | Cloning in pWS3 |
| 1228_U | CCGGGGATCCGAATTCAAAATCATGGATTACATTTTCTC | TCGTCGTTACTGCTACAGACAAATCCCAATACCAA | Cloning in pWS3 |
| 1228_D | TAGCAGTAACGACGATCGT | GCAGGTCGACGAATTCAAATCTGCTTGTCTTCTGCA | Cloning in pWS3 |
| pWS3 | TTTCCCAGTCACGACGTTGT | ACTGACAGCTTCCAAGGAGC | Cloning verification |
| Tet(S) | GACTGTGAATCTAAATTTGAAACC | GCACAATTTCGTGAGTTACTGT | PCR detection |
| Tet(T) | TAGCACATGTTGATGCAGGT | TATCATCCCTTACATTTGTC | PCR detection |
| Tet(U) | CAAAAGAAATCGATACGTGG | CGTCTGCAGATTCCTTAAAAGTC | PCR detection |
| Tet(L) | CATTTGGTCTTATTGGATCG | ATTACACTTCCGATTTCGG | PCR detection |
| Tet(M) | ACAGAAAGCTTATTATATAAC | TGGCGTGTCTATGATGTTCAC | PCR detection |
| Tet(K) | TATTTTGGCTTTGTATTCTTTCAT | GCTATACCTGTTCCCTCTGATAA | PCR detection |
| Tet(O) | GATGGCATACAGGCACAGAC | CAATATCACCAGAGCAGGCT | PCR detection |
| Tet(X) | CAATAATTGGTGGTGGACCC | TTCTTACCTTGGACATCCCG | PCR detection |
a
The underlined sequences correspond to restriction enzyme recognition sites (BamHI, GGATCC; KpnI, GGTACC; EcoRI, GAATTC).