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Time-kill kinetics of vancomycin (100 μg/ml), trimethoprim (0.15 μg/ml), and the two drugs combined in wild-type E. coli compared to that of a no-drug control consisting of LB medium only. Cultures were started with an inoculum of 1 × 104 cells/ml, and viable cells were measured by plating every 2 h for 8 h. Viable cell counts were plotted against time durations on a semilogarithmic graph.
