TABLE 2.

Amino acid residue variations between GSK2251052-resistant E. coli urine isolates and the baseline urine isolate from subject 252 determined by whole-bacterial-genome DNA sequencing

Time(s) of sample collection	Gene	Description	Mutation(s)a
Day 13	leuS	Leucyl-tRNA synthetase	D298N (D251N)
Days 2, 4, 8, and 13	leuS	Leucyl-tRNA synthetase	T294I (T247I)
Day 8	leuS	Leucyl-tRNA synthetase	A370V (A323V)
Follow-up	leuS	Leucyl-tRNA synthetase	D392A, R391H (D345A, R344H)
Days 2, 4, 8, and 13 and follow-up	acnB	Bifunctional aconitate hydratase	V705I
Days 2, 4, and 8 and follow-up	c0279	Hypothetical protein	T21A
Day 4	c1464	Tail fiber component K of prophage	G41b
Follow-up	c1472	Hypothetical protein	T15A
Follow-up	c1473	Hypothetical protein	R8G, 42Eb
Follow-up	c1474	Hypothetical protein	K209Q
Day 2	c3157	Hypothetical protein	T152A
Days 2 and 4 and follow-up	c3157	Hypothetical protein	A158V, W159S, P218L
Day 2 and follow-up	c3157	Hypothetical protein	R136L, D142G
Day 8	c3157	Hypothetical protein	W159S
Days 8 and 13	c3157	Hypothetical protein	T152A, A158V, P218L
Days 2, 8, and 13	c3158	Tail component of prophage	Y85H
Follow-up	c3670	Hypothetical protein	E148G
Follow-up	c3671	RadC-like protein YeeS	L13P
Days 2, 4, 8, and 13	c3674	Hypothetical protein	A160P
Days 2 and 4 and follow-up	c3679	Hypothetical protein	C13Y, L17S
Day 4	c4492	ShiA-like protein	K302Q, E305K
Day 2 and 8	c4572	Hypothetical protein	P98H
Days 2, 4, 8, and 13 and follow-up	c5158	Hypothetical protein	S30,b I22V
Day 8	c5213	Transposase IS629	207K,b H203Y
Days 4, 8, and 13 and follow-up	ompR	Osmolarity response regulator	R207H
Days 2, 4, 8, and 13 follow-up	ycbS	Outer membrane usher protein YcbS	M315I
Follow-up	ydfR	Hypothetical protein	M59R

^aAmino acid residue according to E. coli CFT073 numbering system, with E. coli K12 equivalents in parentheses.

^bNonsense mutation.