TABLE 5.

MICs of E. coli strains with topoisomerase target mutations^a

Strainb	MIC (μg/ml)			Mutation(s)
	NBTI 5463	Ciproflox.	Levoflox.	GyrA	ParC	ParE
W3110	0.5	0.03	0.06
AZ480	1	0.015	0.03	D82E
AZ481	0.5	0.06	0.125	D82G
AZ484	8	0.03	0.03	D82E	K277N, K278L
AZ486	32	0.03	0.06	D82E		P439Q
AZ489	64	0.125	0.25	D82G	D79G
AZ699	0.5	0.03	0.03		K277N, K278L
AZ727	0.5	0.03	0.03		D79G

^aFor all strains in the table, all four topoisomerase genes (gyrA, gyrB, parC, and parE) were sequenced.

^bAZ484 was isolated at 2× MIC (2 μg/ml), AZ486 was isolated at 4× MIC (4 μg/ml), and AZ489 was isolated at 16× MIC (8 μg/ml).