FIG 1.

Electrophoretic profiles of plasmids (a), hybridization with a bla_KPC-2-specific probe (b), and plasmids digested with EcoRI and HindIII (c). (a and b) In lanes 1, 3, and 5, plasmids were extracted from parental isolate K. pneumoniae HS092839, E. coli HS102707, and E. aerogenes HS112625, respectively; in lanes 2, 4, and 6, plasmids were extracted from E. coli J53 HS092839 transconjugant, E. coli J53 HS102707 transconjugant, and E. coli J53 HS112625 transconjugant, respectively. The asterisk in panel b indicates a faint bla_KPC-2-positive band. (c) In lanes 1, 2, and 3, plasmids derived from isolates K. pneumoniae HS092839, E. coli HS102707, and E. aerogenes HS112625 were digested with EcoRI; in lanes 4, 5, and 6, plasmids derived from isolates K. pneumoniae HS092839, E. coli HS102707, and E. aerogenes HS112625 were digested with HindIII.