FIG 3.

Electron microscopic assessment of L. donovani promastigotes treated with free and SSG-encapsulated PC-DDAB. (A) Control promastigotes demonstrate an elongated body and a normal aspect of the intracellular organelles. (B) The 75% effective dose (ED₇₅) of PC-DDAB induced vacuolization and alterations at the plasma membrane. (C) Parasites treated with the ED₇₅ of PC-DDAB incorporating SSG were spherical, with a complete absence of electron-dense materials. Bars, 2 μm (magnification, ×30,000) (A and B) or 1 μm (magnification, ×60,000) (C). Nu, nucleus; Mt, mitochondria; K, kinetoplast; FP, flagellar pocket; DMt, dilated mitochondria; FN, fragmented nucleus; V, vacuoles; dM, dilated matrix of mitochondria; G, granules; MB, membrane blebbing.