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Sequences of forward and reverse primers as used in the PCR step and the expected fragment sizes
| Primera | Sequence (5′ to 3′) | Amplicon size (bp) |
|---|---|---|
| Pfmdr1-FW1 | ACA-AAA-AGA-GTA-CCG-CTG-AAT | 534 |
| Pfmdr1-RV1 | AAA-CGC-AAG-TAA-TAC-ATA-AAG-TC | |
| Pfmdr1-FW4 | TCG-TTG-GAG-AAA-CAG-GTA-GTG | 359 |
| Pfmdr1-RV4 | CAA-TGT-TGC-ATC-TTC-TCT-TCC | |
| Pfcrt-FW | ACG-AGC-GTT-ATA-GAG-AAT-TAG | 422 |
| Pfcrt-RV | GTT-GTG-AGT-TTC-GGA-TGT-TAC |
FW, forward primer; RV, reverse primer.
