TABLE 2.
Labeled primer sequences in the PEXT reactionsa
| Primer | Sequence (5′ to 3′) | 5′ tagb |
|---|---|---|
| Pfmdr1-86N | GGA-TTA-ATA-TCA-TCA-CCT-AAA-TT | Digoxigenin |
| Pfmdr1-86Y | GGA-TTA-ATA-TCA-TCA-CCT-AAA-TA | TEG-dinitrophenol |
| Pfmdr1-184Y | AAA-AAA-AAA-AGC-ATT-TTT-TAT-TAA-TGA-CCA-AAT-AT | Texas Red |
| Pfmdr1-184F | AAA-AAA-AAA-AGC-ATT-TTT-TAT-TAA-TGA-CCA-AAT-AA | 6-FAM |
| Pfmdr1-1246D | AAA-AAA-AAA-AAA-AAA-AAC-TAT-TGA-AAA-TAA-GTT-TCT-AAG-ATC | Cy5 |
| Pfmdr1-1246Y | AAA-AAA-AAA-AAA-AAA-AAC-TAT-TGA-AAA-TAA-GTT-TCT-AAG-ATA | Alexa Fluor 488 |
| Pfcrt-76K | AAA-AAA-AAA-AAA-AAA-AAT-TTT-GTT-TAA-AGT-TCT-TTT-AGC-AAA-AAT-TT | Digoxigenin |
| Pfcrt-76T | AAA-AAA-AAA-AAA-AAA-AAT-TTT-GTT-TAA-AGT-TCT-TTT-AGC-AAA-AAT-TG | TEG-dinitrophenol |
a
Different 5′ tags were used for the detection of each genotype in a codon to discriminate the wild-type and the mutant genotype on the NALFIA strip.
b
TEG, triethylene glycol; FAM, 6-carboxyfluorescein.