FIG 1.

The mouse-to-mouse model. A schematic illustrating the experimental design of the mouse-to-mouse model using P. berghei and A. stephensi. For each treatment group, the drug was administered to five P. berghei-infected mice which were used to feed a cage of 500 female A. stephensi mosquitoes 24 h later. After 10 days, oocyst intensity and prevalence were determined. When sporozoites were maximally infectious (21 days after feeding [28]), individual naive mice were bitten by either 2, 5, or 10 mosquitoes (the mosquito biting rate [MBR]). Salivary glands were dissected postbite to determine sporozoite intensity and prevalence. The presence of infection in the peripheral blood of challenged naive mice (i.e., the number of secondary malarial infections) was monitored daily for 10 days postbite, with parasitemia, gametocytemia, and time to patency recorded.