FIG 2.
Expression of hybrid zinc cluster transcription factors in C. albicans. (A) General structure of the cassettes used for expression of hybrid transcription factors. The DNA-binding domains of the transcription factors are represented by the light gray box. PADH1, ADH1 promoter; TACT1, transcription termination sequence of the ACT1 gene; caSAT1, Candida-adapted nourseothricin resistance marker; 3′ADH1, sequence from the 3′ part of the ADH1 gene. The flanking ADH1 sequences (PADH1 and 3′ADH1) served for integration into the ADH1 locus by homologous recombination. (B) Schematic diagrams of the hybrid transcription factors constructed in this study. Mrr1, Tac1, and Upc2 sequences are represented in blue, red, and green, respectively. The extent of the DNA-binding domain (DB) and the remainder of the proteins are indicated (amino acid positions correspond to those in the wild-type transcription factors). The Gly-Ala linker that was inserted between the two parts of each protein is also shown.