TABLE 1.
Open reading frames on the S. aureus chromosome encoding putative efflux proteins
| Open reading frame in strain: |
Genea | Protein length (amino acids) | TMSb | Predicted protein family and substrate(s)c | Reference(s) | ||
|---|---|---|---|---|---|---|---|
| NCTC 8325 | COL | N315 | |||||
| 0042 | 0070 | NPd | 406 | 12 | MFS, macrolides | ||
| 0058 | 0086 | 0099 | norC | 462 | 14 | MFS, multidrug | 12 |
| 0062 | 0090 | 0103 | 290 | 10 | DMT, drugs/amino acids | ||
| 0078 | 0103 | 0115 | 418 | 12 | MFS, multidrug | ||
| 0099 | 0122 | 0132 | tet38 | 450 | 14 | MFS, tetracycline | 11 |
| 0143 | 0163 | 0172 | 416 | 12 | MFS, multidrug | ||
| 0246 | 0261 | 0263 | 458 | 14 | MFS, quinolones | ||
| 0315 | 0405 | 0323 | mepA | 451 | 12 | MATE, multidrug | 9 |
| 0556 | 0620 | 0531 | 466 | 12 | MFS, proline/betaine | ||
| 0681 | 0733 | 0628 | 406 | 12 | MFS, sugars | ||
| 0703 | 0754 | 0650 | norA | 388 | 12 | MFS, multidrug | 13, 14 |
| 0740 | 0790 | 0684 | 244 | 8 | DMT, drugs/amino acids | ||
| 0952 | 1021 | 0874 | 402 | 12 | MFS, glycolipids | ||
| 1448 | 1475 | 1269 | norB | 463 | 14 | MFS, multidrug | 11 |
| 1876 | 1809 | 1580 | 393 | 12 | MFS, multidrug | ||
| 2418 | 2157 | 1970 | lmrS | 480 | 14 | MFS, lincomycin/multidrug | 15 |
| 2419 | 2158 | 1971 | sepA | 157 | 4 | Novel,e multidrug | 16 |
| 2420 | 2159 | 1972 | sdrM | 447 | 14 | MFS, multidrug | 17 |
| 2435 | 2170 | 1982 | 397 | 12 | MFS, macrolides | ||
| 2525 | 2252 | 2056 | 1,055 | 12 | RND, multidrug, AcrB-like | ||
| 2531 | 2257 | 2061 | 403 | 12 | MFS, drugs | ||
| 2629 | 2347 | 2142 | 643 | 15 | MFS, lincomycin/multidrug | ||
| 2700 | 2413 | 2203 | mdeA | 452 | 14 | MFS, multidrug | 8 |
| 2725 | 2437 | 2222 | 403 | 12 | MFS, bicyclomycin/teicoplanin | ||
| 2740 | 2449 | 2233 | 466 | 14 | MFS, quinolones/multidrug, NorB-like | ||
| 2752 | 2460 | 2241 | 375 | 12 | MFS, chloramphenicol/multidrug | ||
| 2762 | 2471 | 2250 | norD | 397 | 12 | MFS, drugs | 18 |
| 2797 | 2504 | 2283 | 397 | 12 | MFS, unknown | ||
| 2818 | 2523 | NP | 387 | 12 | MFS, multidrug | ||
| 2843 | 2548 | 2322 | 311 | 10 | DMT, drugs/amino acids | ||
| 2866 | 2566 | 2339 | 822 | 12 | RND, multidrug, MmpL-likef | ||
a
If previously characterized.
b
TMS, transmembrane segments. Secondary structure predictions of putative pump proteins were made by the transmembrane helix prediction algorithm TMHMM at www.cbs.dtu.dk/services/TMHMM.
c
Predicted by TransAAP and PSI-BLAST tools available at www.ncbi.nlm.nih.gov. MFS, major facilitator superfamily; MATE, multidrug and toxic compound extrusion; DMT, drug/metabolite transport; RND, resistance-nodulation-division.
d
NP, not present within the genome.
e
Structurally dissimilar to known pump families.
f
MmpL, Mycobacterial membrane protein large, involved in mycolic acid and lipid transport (19).