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The 30-h drug susceptibility assay using strain P. falciparum 3D7A. The dependency of growth inhibition on the coimport of Gb-2.44 (●) was determined by adding the fusion protein and the unrelated fusion Gb-H22 (□) or nonfused Gb (⊗) to synchronous ring-stage parasites and incubating them for 30 h, when mature schizonts but no rings were present to exclude the potential coimport step. The data represent the mean ± SD from two experiments, using technical triplicates.
