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. 2014 Dec 23;59(1):527–535. doi: 10.1128/AAC.03818-14

FIG 4.

FIG 4

Binding of R33 and bvR33 VHH to surface-expressed gD2 on z4/6 cells. z4/6 cells (surface expression of gD2) were stained with various VHH (R33, P10, and bvR33) and detected using fluorescence-activated cell sorting (FACS) by a FITC-conjugated anti-His secondary antibody, demonstrating that R33 and bvR33 but not P10 can bind to gD2. DL6 was used as a positive control to verify that gD2 was expressed, and a secondary antibody control (anti-His) with no VHH or primary antibody was also used as a negative control.