FIG 5.

HSV-2 neutralization assay. Virus was incubated with dilutions of R33 and HSV8 (known neutralizing antibody) (A) or R33, bvR33, and P10 (B) and then plated on Vero cells to assay for HSV-2 neutralizing activity. While HSV8 is capable of neutralizing HSV-2 (IC₅₀ of 1.5 nM), R33 and bvR33 do not differ from P10 in terms of inhibiting viral infection. Each dilution was assayed in duplicate, and error bars represent maximum and minimum plaque numbers. These graphs are representative of at least three independent experiments, and results are expressed as percent inhibition compared to plaque numbers from untreated virus. Statistical significance compared to results for untreated virus was calculated by ANOVA and is indicated by asterisks (P < 0.05).