Table 1. Summary of Kar5p experiments.
| GFP Expression | SPB Enrichment | Prm3p Recruitment | Fusion | Function | |
|---|---|---|---|---|---|
| KAR5 | + | ++ | + | + | |
| SP | − | +/− | − | − | Stability |
| Conserved | ++ | − | +/− | − | SPB interaction |
| Coil1 | + | + | + | − | Fusion? |
| Coil2 | ND | ND | − | − | ? |
| TM1 | + | +/− | − | +/− | Prm3p recruitment |
| TM2 | + | + | − | − | |
| Loop-TM2 | ++ | + | − | − | |
| TM1-loop-TM2 | + | + | +/− | − | |
| C13A | +/− | + | +/− | +/− | Stability |
| C56A | +/− | +/− | +/− | − | ? |
| C68A | + | + | + | − | Envelope link |
| C91A | + | − | +/− | − | SPB interaction? |
| C105 | + | − | +/− | − | SPB interaction? |
| C116A | + | − | + | − | SPB interaction? |
| C141A | + | + | − | − | Prm3p recruitment? |
| C444A | ND | ND | + | + | |
| C458A | ND | ND | + | + |
Phenotype levels from highest to lowest are ++, +, +/−, and −. ND, no data. Raw P-values associated with this table are shown in Table S2. GFP, green fluorescent protein; SPB, spindle pole body.