Figure 3.

The y11-2 and y11 mutations in the candidate gene Glyma13g30560 appear to be novel de novo mutations. (A) Gene diagram for the candidate gene Glyma13g30560: the 3 exons are indicated in blue, and 5′ untranslated region (UTR) and 3′ UTR are in gray. (B) Sequence results of MinnGold (y11-2) and T219 (y11) compared with a panel of diverse soybean lines. Of the 29 diverse soybean lines sequenced (15 are shown), only y11-2 was found to have adenine rather than guanine at position 33146523 and only y11 was found to have the guanine rather than the adenine at position 33146529. The y11-2 SNP causes a nonsynonymous change from arginine to glutamine, and the y11 SNP causes a nonsynonymous change from glutamine to arginine.