Figure 1.

Mutation of Pdk1 alters the developmental response to ethanol. (A) Pdk1^EP3091 mutant flies are sensitive to ethanol-induced developmental delay. The red line indicates 50% eclosion. Times to 50% eclosion were: WT 0% EtOH, 11.85 ± 0.26 d; WT 6% EtOH, 13.05 ± 0.24 d (not significant, one-way ANOVA with Tukey HSD post hoc analysis); Pdk1^EP3091 0% EtOH, 12.76 ± 0.51 d (not significant, one-way ANOVA with Tukey HSD posthoc analysis); and Pdk1^EP3091 6% EtOH, 14.75 d ± 0.25 d (P < 0.01 vs. both 0% controls, one-way ANOVA with Tukey HSD post hoc analysis). Data are normalized to the total number of flies that eclosed for that treatment condition and genotype. (B) Pdk1^EP3091 mutant flies are resistant to ethanol-induced developmental lethality. Survival has been normalized to age- and genotype-matched controls reared in ethanol-free food. N = 4; P = 0.044, Student’s t-test.