Figure 4.

Ethanol-reared flies show increased lipid storage and starvation resistance. (A) Confocal reconstruction of a Nile Red–stained fat body dissected from a wandering third instar larva that was reared in control food containing no ethanol. (B) Confocal reconstruction of a Nile Red–stained fat body dissected from an ethanol-reared wandering third instar larva. Note the increased diameter of fat droplets relative to the control fat body shown in (A). (C) Adult flies reared in ethanol show increased resistance to starvation, with median survival of an additional 1.5 d on 1% agarose medium, which provides water but no nutrients. Median survival of control flies: 2.3 d; median survival of ethanol-reared flies: 3.8 d; P < 0.0001, Student’s t test, N = 10.