Table 3. Larval gene expression changes in response to ethanol.
| Gene | Expression Change | Protein Function | Predicted Effect | |
|---|---|---|---|---|
| Oxidative stress-related | Cyp4e3 | 121-fold increase | Cytochrome P450 | Increased ROS |
| Cyp6a2 | 4.2-fold increase | Cytochrome P450 | Increased ROS | |
| CG32750, CG32751, vanin-like | 3.6- to 12.5-fold increases | Pantetheine hydrolases | Increased sensitivity to ROS/increased fatty acid synthesis | |
| Cyp4d21 | 6.7-fold decrease | Cytochrome P450 | ||
| GSTD8 | 4.6-fold decrease | Glutathione-S-transferase | Increased ROS | |
| GSTD4 | 4.3-fold decrease | Glutathione-S-transferase | Increased ROS | |
| Lipid metabolism | ||||
| Gpdh | 4.1-fold increase | Glycerol-3-phosphate dehydrogenase | Fatty acid accumulation | |
| GlcT-1 | 4-fold increase | Ceramide glucosyltransferase | Fatty acid accumulation | |
| Acox57D-d | 3.9-fold increase | Acyl-coenzyme A oxidase | Fatty acid accumulation | |
| Gpo-1 | 3.2-fold increase | Mitochondrial glycerol-3-phosphate dehydrogenase | Fatty acid accumulation | |
| CG17191, CG17192, CG6271, CG6277, CG6283, CG6295, CG8093 | 5.1- to 216-fold decreases | Triglyceride lipases | Fatty acid accumulation | |
| CG31148, CG31414, CG33090 | 6.4- to 11-fold decreases | Glucosylceramidases | Fatty acid accumulation | |
| aSMase | 9.9-fold decrease | Acid Sphingomyelinase | Reduced ceramide | |
| ATPCL | 8.3-fold decrease | ATP citrate lyase | Possible increased storage of triglycerides |
RNA was isolated from third instar wandering larvae reared in either 0% or 7% ethanol, and gene expression was compared using Affymetrix Drosophila 2.0 microarray chips. Data are presented as fold-change in 7% reared larvae relative to control larvae (developing in 0% ethanol).