Figure 5.

H3K9me3 inhibits WC-2 binding. WC-2 binding at the (A) PLRE and (B) aLRE promoter elements was determined by ChIP of DNA from WT (FGSC2489) and Δdim-5 strains exposed to light for the indicated times. Δwc-2 and a nonspecific IgG were used as controls. The data are averages of four independent experiments and the asterisk indicates times at which greater levels of WC-2 are found associated with the pLRE or aLRE. The error bars represent the SEM with P < 0.05. ChIP, chromatin immunoprecipitation; pLRE, proximal light−regulated element. aLRE, antisense light-regulated element.