Skip to main content
. 2014 Nov 14;34(1):115–129. doi: 10.15252/embj.201489559

Figure 5. Proliferation of cay1Δ cells.

Figure 5

  • A Serial dilutions of the indicated strains were spotted on complete medium and grown at 30°C or 20°C. Otrt1Δ and Otrt1Δcay1Δ are strains with circularized chromosomes lacking telomeric sequence (Supplementary Fig S7).
  • B Representative images of wt and cay1Δ cells grown for 24 h at 30°C or 20°C. Scale bar, 20 μm.
  • C Quantification of cell length for strains grown in complete medium for 24 h at 30°C or 20°C. Each dot represents a single cell and median cell length (m) is indicated for each sample. Data are from three independent experiments and at least 100 cells were scored per sample in each experiment.
  • D Top: Examples of DAPI-stained wt and cay1Δ cells grown in complete medium for 24 h at 30°C or 20°C. The white arrowhead indicates a chromosome bridge. Scale bar, 10 μm. Bottom: Quantification of chromosome bridges. Bars and error bars are averages and s.d. from three independent experiments and at least 200 cells were scored per sample in each experiment. Statistical significance was assayed using the unpaired, two-tailed Student's t-test. *P < 0.001 relative to 30°C.