Figure 2.

Fra-1 expression in EpH4 cells leads to fibroblastoid conversion and increased cell motility and invasiveness in vitro. (a) Analysis of Fra-1 protein (Top) and mRNA (Bottom) in EpH4-derived cells. Heterochromatin Protein 1a (Hp1α) is used to control loading. Expression in parental EpH4 is set to 1. (b) Cell morphology of EpH4-derived cells. (c) Western blotting and (d) qRT-PCR analysis of epithelial and mesenchymal markers in EpH4-derived cells. Actin and Tubulin are used to control loading in panel (c), and EpH4 are set to 1 in panel (d). Bars=mean±S.D., n=2. In vitro (e) proliferation, (f) migration and relative invasion (g) of EpH4-derived cells. Bars=mean±S.D., n=3. cdh1, cdh2, cdh3, fn1, vim, ctnna1, ctnnb1, jup and ctnnd1 encode for E-cadherin, N-cadherin, P-cadherin, Fibronectin (Fn), Vimentin, α-catenin (α-ctn), β-catenin (γ-ctn) and p120-catenin (p120ctn), respectively