Figure 4.

Allogeneic HCT mice transplanted with CCL2^−/− or CCR2^−/− donors have reduced pulmonary CCL2 and CCR2, respectively. Mice underwent an allogeneic HCT using wild-type (WT), CCL2^−/−, or CCR2^−/− donors and were killed without LPS exposure and at 4 and 72 hours after 5 days of daily LPS exposures. Allo mice transplanted with CCL2^−/− donors have reduced CCL2 protein in their BAL (A) but have unchanged CCL2 (B) and CCR2 transcripts (C) in the lung tissue. Allo mice transplanted with CCR2^−/− donors have elevated CCL2 protein in their BAL (A), unchanged CCL2 transcripts in the lung tissue (B), and reduced CCR2 transcript in the lung (C). Data represent the average ± SEM and have been replicated in two independent experiments. ⁺P < 0.05 comparing CCL2^−/− group with WT. *P < 0.05 comparing CCR2^−/− group with WT.