Figure 5.

Allogeneic HCT mice transplanted with CCL2^−/− or CCR2^−/− donors have reduced pulmonary DCs. Mice underwent an allogeneic HCT using WT, CCL2^−/−, or CCR2^−/− donors and were killed without LPS and at 4 and 72 hours after 5 days of daily LPS exposures. BAL cells were analyzed via flow cytometry. (A) Allo mice transplanted with either CCL2^−/− or CCR2^−/− donors have only a trend toward reduced inflammatory monocytes in their BAL. (B and C) Allo mice transplanted with CCL2^−/− donors have a significant reduction in overall DC (B) and CD11b⁺ DC (C), but not in moDC numbers (C). (B–D) Allo mice transplanted with CCR2^−/− donors have a significant reduction in the overall DC, CD11b⁺ DC, and moDC numbers in their BAL. Data represent the average ± SEM and have been replicated in two independent experiments. ⁺P < 0.05 comparing CCL2^−/− group with WT. *P < 0.05 comparing CCR2^−/− group with WT.